CRISPR/Cas9 targeting events cause complex deletions and insertions at 17 sites in the mouse genome. CRISPR off-target detection with DISCOVER-seq. The next generation of CRISPR-Cas technologies and applications. Technologies and computational analysis strategies for CRISPR applications. Cornerstones of CRISPR-Cas in drug discovery and therapy. CRISPR-based technologies for the manipulation of eukaryotic genomes. ![]() Biology and applications of CRISPR systems: harnessing nature’s toolbox for genome engineering. Development and applications of CRISPR-Cas9 for genome engineering. The entire protocol from genomic DNA extraction to OnTE exclusion can be performed in 6–9 d. All steps are optimized to maximize simplicity and minimize cost to promote wide dissemination and applicability across the field. Second, occurrence of LOH around the edited locus is detected by genotyping neighbouring single-nucleotide polymorphisms (SNPs), using either a Sanger sequencing-based method or SNP microarrays. This combination of genotyping and quantitation makes it possible to exclude clones with monoallelic OnTEs and hemizygous editing, which are often mischaracterized as correctly edited in standard Sanger sequencing. This is achieved by subjecting genomic DNA first to quantitative genotyping PCR (qgPCR), which determines the number of intact alleles at the target site using the same PCR amplicon that has been optimized for genotyping. ![]() Our protocol enables identification of OnTEs such as large deletions, large insertions, rearrangements or loss of heterozygosity (LOH). Here we describe a broadly applicable framework for detecting OnTEs in genome-edited cells and organisms after non-homologous end joining-mediated and homology-directed repair-mediated editing. By altering gene expression of targeted or neighbouring genes, OnTEs can severely affect phenotypes of CRISPR-edited cells and organisms and thus lead to data misinterpretation, which can undermine the reliability of CRISPR-based studies. However, recent reports indicate widespread occurrence of unintended CRISPR-induced on-target effects (OnTEs) at the edited site in mice and human induced pluripotent stem cells (iPSCs) that escape standard quality controls. The recent CRISPR revolution has provided researchers with powerful tools to perform genome editing in a variety of organisms. Override the reading frame saved with the. Override the minimum ORF length saved with the. Override enzyme set to use saved with the. “ “.Īdds mouse handlers in the hittest region of the DOM. To clear the title completely from display, set the value to one space, e.g. If the subtitle is not defined or set to an empty string, then the default title (the number of base pairs) is used. “ “.ĭefines the subtitle to use for the map. If the title is not defined or set to an empty string, then the default title (the filename without the extenion) is used. ![]() Useful when supporting multiple maps or sequences on the same page.ĭefines the title to use for the map. User defined attribute used to identify a particular map or sequence view. Useful when supporting multiple maps or sequences on the same page Removes extra SVG attributes that are not necessary for correctly rendering the SVG. Instead use the JS file in /opt/gslbiotech/snapgene-server/examples/js/map-seq-interactivity.jsįile to text fragment of the javascript code required for hover/selection (dynamic part).įile to text fragment of the css code required for proper rendering of the SVG DOM. NOTE: this parameter is deprecated - there is no need to generate JS files using generateSVGMap. The actual size might be different because the map generation code performs extra logic to ensure the text graphical elements are all visibleįile to text fragment of the svg objects and tooltip DOM objectsįile to text fragment of the javascript code required for hover/selection (static part) the static part can be shared for all maps for the same version of snapgene server. Set the requested size of the map in width x height. ![]() True for linear map, false for circular map
0 Comments
![]() This type of template includes a blank space for you to enter the name of the product or service sold, the price sold for, and any relevant taxes paid. Generally speaking, there will be two copies issued at the point of sale - one for the customer and one for the seller or vendor. It’s just a printed acknowledgment that shows the amount of cash - or cash equivalent - that was received during a transaction. Cash receipt templateĪ cash receipt is one of the most basic receipt types. But to help you wrap your head around the basics, we’ll walk you through a few of the most common receipt templates you’re likely to need when doing business. There are many different kinds of receipts - and just as many free receipt templates with varying template designs. What are some examples of receipts templates? A Work OS that offers workflow automations and can tag each receipt as it comes in can help you take back control of your bookkeeping. Microsoft Word or Google Docs receipt templates are great, but they can be hard to manage at scale. ![]() Using an online template makes it easier for a distributed team of accountants or tax relief experts - like between offices - to coordinate and get their numbers right. This includes cash register tapes, deposit information from cash and credit sales, receipt books, petty cash slips, and invoices. The Internal Revenue Service (IRS) requires you to show documentation of certain expenses, and it recommends small businesses keep all gross receipts. Templates also help standardize your receipts and help your accounting team with their tax filings. ![]() With the help of a receipts template, you can drastically reduce the amount of time you spend filling out customer receipts because all of the structure and recurring content have already been taken care of. You should use receipts templates because they help you save time, simplify accounting, and set your business up for growth. ( Image Source) Why use receipts templates? One benefit of a receipt template is the ability to use embedded formulas, so you don’t manually have to calculate things like value-added tax - we’ll cover a few more reasons why you should use a receipt template next. ![]() Most templates will allow you to customize your receipts to add materials like brand names, company icons, logos, or even change the color palette. But you might also get a PDF receipt or even a basic Microsoft Word document.Ī receipt template typically includes basic fields to collect important information about these transactions, like date, subtotal, taxes, unit price, company details, information about each sale, and more. After all, receipts can take many shapes - including email or print receipts for card or cash payments. But even B2B (business-to-business) transactions and deals rely on receipts to maintain a paper trail.Ī receipt template is a pre-built receipt that business owners and individuals can customize to fit a variety of transactions. You probably get at least 10 receipts a day from various stores. Get the template What is a receipts template?Ī sales receipt gets issued by vendors and service providers to customers and serves as proof of payment. They give customers the power to prove they’ve paid for something, and they give businesses the ability to prove goods or services have been rendered.Īs a business owner, or any process manager for that matter, you know just how important it is to streamline the receipts process and have a better handle on what’s going in and out of your organizations In this article, we’ll provide our own receipts template, showcase different use cases, and explain how you can use it effectively. If you’ve ever bought something and wish you hadn’t, you’ll know why receipts are so important. ![]() The TSS Handbrake Sparco Mod + features Thrustmaster’s H.E.A.R.T HallEffect AccuRate Technology for unrivaled precision, and an unlimited product lifespan.The gear knob is an exact replica (1:1 scale, 3.3”/8.5 cm tall) of the genuine gear knob from Sparco’s Rally and drifting range, for incredible realism.This new Thrustmaster product is the must-have accessory for optimal racing simulation on game consoles and PC. Compatible on game consoles with the T-GT and T300-Series racing wheel bases on PS4* and with the TS-XW Racer and TX-Series racing wheel bases on Xbox One*, the TSS Handbrake Sparco Mod + is also compatible on PC with all racing wheels available on the market. The TSS Handbrake Sparco Mod + is a two-in-one product with two different modes: progressive handbrake mode, and sequential shifter mode.The TSS Handbrake Sparco Mod + is a 1:1 scale replica of a genuine shifter: its extreme realism will fully immerse you in the world of racing! Officially licensed by Sparco, it allows for two different modes (progressive handbrake, and sequential shifter), for optimal use in all compatible games*. The newest product resulting from the collaboration between Thrustmaster and Sparco, this accessory joins Thrustmaster’s racing ecosystem on game consoles. Following in the footsteps of the PC-compatible TSS Handbrake Sparco Mod, Thrustmaster proudly presents the TSS Handbrake Sparco Mod +: compatible with PS4*/Xbox One* and PC. ![]() Stop struggling with your putty scraping needs and get the job done right with Harrier Soft Grip Handle Putty Scrapers! This four-pack gives you more bang for your buck, so you can tackle any project with ease. Get yours today and give any putty job the precise touch that only a high-quality tool can provide! Even better, it's backed by an Amazon Basics 1-year limited warranty for additional peace of mind. ![]() Thanks to its classic-style handle, you can enjoy a safe, sturdy hold along with a professional look and feel every time you use it. Plus, its flexible blade is specially taper-ground and mirror-polished carbon steel, making it an ideal choice for spreading spackle and adhesive evenly. The Amazon Basics 2" Flex Nylon Handle Putty Knife's durable construction of 55% carbon steel and 45% nylon makes it perfect for heavy-duty projects. Whatever kind of project you’re tackling next - be it nailing walls or putting down flooring - Mister Rui’s Putty Knife offers a reliable, multifunctional tool to get you done right – fast! Plus it’s guaranteed solvent-resistant so you can use it time after time without a sweat. ![]() With a PP + TPR ergonomic handle for greater control and comfort, you’ll be pressing through projects faster and with less strain on your hands. The rust-proof stainless steel blade is made for precision ground cutting and will make light work out of any job. It's perfect for applying and removing putty, shoveling paint and glue, pulling nails, opening cans, and cleaning rollers. The Mister Rui Putty Knife, 10-in-1 Multi-Use Painters Tool crafted with high-quality materials, this paint scraper is designed to last longer and work more efficiently. Whether you're working on drywall spackle in tight corners or sanding fine details in large areas, this WORKPRO set has you covered! Plus, with its long-lasting durability and resistant nature against corrosion, it will be providing impeccable service for years to come.īest for Home Improvement: Mister Rui Putty knife 10-1 It’s multi-purpose too! The four different width sizes of 1.5”, 3”, 4”, and 6” provide flexibility to tackle difficult projects. Furthermore, the ergonomic PP&TPR handles make these knives soft on your hands while providing a secure grip to ensure safety when using. Highly quality made with durable mirror polished blade and rust resistance means you can use it over and over again without worry. The WORKPRO 4 Piece Putty Knife Set stainless steel set is perfect for repairing walls, taping, spackling, and scraping paint. Plus, it features a large hang-hole size that makes storage and retrieval a breeze!īest for repairing walls: WORKPRO W004902A And thanks to its hammer cap handle, you can feel confident in your task. Greetings DIYers and hobbyists! Are you looking for the perfect putty knife to tackle your next project? Look no further than the Warner 1-1/2” ProGrip Stiff Putty Knife, Stainless Steel with Hammer Cap, 90661.Ĭrafted from high-grade stainless steel, it’s designed with a stiff blade that won’t bend even when facing the toughest challenges. Best for the perfect putty knife to tackle your next project: Warner 90661 ![]() This earth I cry is not my home, I seek my home in heaven. How happy is every child of grace, that knows his sins are forgiven. Please continue to pray for the Kellogg family. The Davis funeral home of Ocilla has charge of all arrangements. Kellogg will lie in state at the Davis funeral home in Ocilla Friday morning 11:00 until 6:00 p.m. Interment will follow in the Ocilla city cemetery, 301 East Valdosta Ave. Minister Marice Lake will deliver the eulogy. from the True-Life Restoration center, 801 North Irwin Ave., Ocilla where Dr. Steven Kellogg is planned for Saturday December 9, 2023, at 11:00 a.m. Odessa Merritt of Ocilla Georgia, along with several grandchildren, brother and sister in laws, aunts, uncles, nieces, nephews, cousins, relatives and friends. Jeanette Walker of Buffalo New York, and Mrs. Alfonso Quinney'Hayes,of Buffalo New York, Ms. Leon Stephenson of Las Vegas Navada, Mrs. Sharika Kellogg of West Palm Beach Florida, Ms. He leaves to cherish his memories his mother: Ms. Kellogg received God's call to his eternal home on Thursday November 30, 2023, at Coffee Regional Medical center in Douglas Georgia. Born October 17, 1955, in Saginaw Michigan, to Ms. Steven Kellogg of 605 South Sherman Street, Fitzgerald Georgia. With sincere sympathy, the Davis funeral home, 301 North Oak Street, Ocilla Georgia, pause to announce the death and home going celebrations for Mr. Let those refuse to sing, who never knew our God, but children of the heavenly king, may speak their joys abroad. Join in a song a sweet accord, and thus around the throne. Please continue to pray for the Barnes and Andrews Families.Ĭome ye that love the lord, and let your joys be known. Lynzettan Nicole Barnes are incomplete at this time and will be announced later by the Davis funeral home of Ocilla. Paula Wilson of Fitzgerald Georgia, along with a host of aunts, uncles, nieces, nephews, cousins, relatives and friends. One grandchild: Malaya Brielle Barnes of Ocilla. Kole Quentavis Barnes all of Ocilla Georgia. Barnes received God's call to her eternal home on Friday afternoon December 15, 2023, at Tift Regional Medical center in Tifton Georgia surrounded by her devoted family. Born October 19, 1983, in Tifton Georgia to Mrs. It is with sadness in our heart, that the Davis funeral home, 301 North Oak Street, Ocilla Georgia 31774 pause to announce the death of Ms. There's a sweet sweet sprit in this place, and I know that it's the presence of the lord. The corporation is headquartered in Ocilla and serves members in Ben Hill, Berrien, Coffee, Irwin, Tift, Turner, Wilcox, and Worth Counties. Irwin EMC was founded in 1936 to supply electricity to people in this rural area. Irwin EMC is a not-for-profit electric utility that supplies energy to around 8,000 members in south central Georgia. Irwin Electric Membership Corporation (EMC) is a service cooperative and is owned by those who receive services (called members). To find out more, please visit our blog at /blog. ![]() on pole attachment issues, including rental rates, permitting, inspection, attacher management, make-ready engineering as well as contract development, negotiation, and administration. McLean Engineering provides design, engineering, and inspection solutions, and is nationally-recognized in the area of pole attachments, having presented to national audiences on the topic and consulted for electric utilities across the U.S. Over the years, McLean Engineering has built a reputation for providing safe, reliable and durable design solutions by focusing on long-term relationships with their clients. McLean works with many municipalities, cooperatives and state trade organizations to navigate through joint use and pole attachment concerns by providing expertise in all areas related to joint use and pole attachment matters.Įstablished in 1936, McLean Engineering has over 85 years of experience in the power engineering field, serving electric utility systems throughout the United States. The cooperative selected McLean Engineering to provide this count in an effort to collect updated information on a GIS basis, improve relationships with its attachers, and clean up late transfers. Irwin Electric Membership Corporation (EMC), based in Ocilla, Ga., has selected McLean Engineering Company to provide a pole attachment inventory for the Cooperative’s nearly 2,000 miles of distribution line over eight counties.Īs part of its joint use and pole attachment agreements, Irwin EMC is due to conduct a pole attachment count and joint use inventory on its system. ![]() Drag the bar further right to expand the width of the sidebar. ![]() There you can adjust the width of the sidebar by dragging the Sidebar Width bar. First, you can move the bar to the left of the desktop by selecting the Dock drop-down menu on the General tab and selecting Left.Ĭlick the Customize tab to open the options shown in the shot below. Click the cog icon at the top of the bar to open the window below. There are a variety of customization options for the sidebar. Drive details highlight how much disk storage you’ve used up. Then there are RAM allocation details that show you your RAM usage. At the top there’s a clock, and just below that are CPU resource details. Your new system diagnostics bar includes a variety of resource details on it. Then open its setup to run through a quick initial setup to open the sidebar in the snapshot below. You can add it to Windows 10 by opening this page and clicking the DOWNLOAD button there. The sidebar shows you RAM, CPU system details and more besides. This is software that adds system details to your desktop with a sidebar. Sidebar Diagnosticsįirst, check out the Sidebar Diagnostics software package. Nevertheless, you can still add system resource monitors to your Windows 10 desktop, taskbar and system tray with a few software packages. However, those system tools don’t show you those details on your desktop. The “ Guide to Windows 10 System Tools” article told you about some of the tools that provide system resource details such as RAM usage. How to add system resource details to the Windows 10 desktop ![]() Would you support them month to month? Probably not. There are tons of non-porn indie games out there, that make less than $100 a month from Patreon, and many of those games are really cool. RisenKill There are multiple issues with this mindset:ġ. Then how about you go ask your Dad why he did that Job? Did he like his Job? Did he have the opportunity to do something else or get a better paid job? Its not your money that he gets, its the money that other people gave him out of their own free will, because they can afford it to give him that money. That money is the result of the work he has been doing on his own for years and keeps on doing. Just because he is a single indie Dev (with an animator that he also has to pay for his work), he does not deserve to get payed as well as someone working for a big Studio? Even tough he literally does (mostly) everything all by himself?Īlso, about those 6000 Dollars/ Month his patreon makes: That money is given to him by people that want to finance this game! He did not wake up one day and say "Oh man, today I want to make 6k a month!". ![]() You will probably just Ignore/ misrepresent what I have to say but I will still say it: You choose to do this all day, crying about a Dev taking the necessary steps to add online features to his game. You would be on the Discord, actually talking to the Dev and not in here, talking about him like you know anything about him.īut you did not want to. If you really would want to help the Game getting made, you would have paid a few bucks at some point. And please, do not go "I will buy it once it is done", the only one you are fooling is yourself. You are sitting here, moaning about everything, while playing the result of the Dev's work and everyone else's money, while not having paid anything for it. You talk about the Dev being greedy, but here you are bitching about everything he does with HIS project, while (and I am certain of this) you have done nothing to support the project. He is also not bitching about it, that is all you people in here, calling forth the one millionth DRM apocalypse, that did not happen (after all, people here are still able to play it with the same old Crack that is now nearly half a year old). ![]() Click to expand.Then how about you go ask your Dad why he did that Job? Did he like his Job? Did he have the opportunity to do something else or get a better paid job?Īgain, it was not the Dev that decided that he gets 6K a month but the collective people who like the game and want it to succeed. ![]() Wood scabbard with quality brass fittings.Ī quality sword demands a quality scabbard! Just like a great car needs a great paint job. Guard and tang cap are made from a quality solid brass. Hand forged 5160 Tempered carbon steel blade, blade sharp. Fully Functional (Battle Ready)! Sword also includes a historical wood scabbard with brass accents. High polished steel spacer at the bottom of the handle for strength and durability. The elite Kingdom of Arms version of this popular Gladius features a hand hammered 5160 carbon waisted steel blade as the originals were made, full tang, heat treated and tempered as the originals, for a strong yet flexible blade that will hold a very sharp edge, with a hard wood handle. The gladius is known as "the sword that conquered the world". Unlike the later Pompeii style, the Mainz pattern was still capable of delivering strong cutting blows, though its primary purpose was for thrusting. The Mainz pattern is deeply waisted and has a long point section like the earlier gladius Hispaniensis, but is shorter and broader than its predecessor. As these battle tactics for the Legions changed, a shorter, broader stabbing/cutting sword had to be developed to use in combination with these newer types of shield and new styles of fighting. Rather than fighting as individual warriors (like their competition, the Continental Celts) the Roman legions eventually developed a new way of fighting - massing together with overlapping shields, using their short stabbing cutting swords to strike from behind this shield wall. All Edged Weapons, Battle Ready or Not, May Injure, Kill and/or Become Damaged if Used Improperly Read About Sword Care. ![]() ![]() Large Battle Ready Medieval Arming Sword. The Mainz pattern Gladii or Gladius is thought to have developed from the leaf-bladed short swords used on the Celt-Iberian Peninsula (what is now Spain) and adopted for use in the Republican Legions. Large Battle Ready Medieval Knightly Sword. It will also show you your savings before checkout. We will be happy to answer your queries as soon as possible within 24 hours.15% off sale, taken off immediately when added to your cart. ![]() If you have any questions or face any problem you can check our FAQs section or feel free to contact us. View medieval sword designs of historical significance for collectors, reenactors, home decor and more Be it a Claymore, Knight Sword, Crusader Sword, our functional battle ready swords and decorative medieval swords dating the middle ages circa AD 500 -1500. Therefore, we will not sell our products to anyone under the age of 18. The blunted flanges and 21 1/2' long steel shaft make this Battle Ready Functional Flanged Mace a good choice for the re-enactor. All swords are handcrafted by skillful artisans and are perfect medieval collectibles. Our products are intended for legal use only by responsible buyers. Buy high-quality battle ready medieval weapons on sale at Swords Knives & Daggers. We are not responsible for any injuries associated with the use of our tools. Never store your sword in leather sheath for a long time, leather can absorb water which will rust the blade. Keep the blade dry after each cleaning and keep oiling to save blade from rust. Take care when removing product from packaging. You can buy this best viking sword battle ready for yourself or for your loved once as their birthday gift, fathers day gift, mothers day gift or wedding gift. This battle ready sword is a handmade viking sword and called functional medieval sword. Our all product are handmade, forged and durable. Gorgeous handle of this viking weapon is made of wire. The blade of this medieval sword with sheath is very sharp and passing through well heat treated temper and hammering process so the blade of this viking sword battle ready is hard enough for use with 58-60 HRC. This real viking sword would look most impressive in any real medieval sword collection. This awesome handmade damascus steel battle ready viking sword designed and custom made by medieval sword maker. Battle Ready For functional weapons Sport Combat For blunted fighting weapons Decorative For display only. So we can do it free (if you have black & white image) for you on the Blade, Handle or Leather Sheath.įor placing Custom or Wholesale orders feel free to Contact us and we promise to respond you as soon as possible within 24 hours. The most medieval of weapons, a flails brutish utility and crushingly powerful concussive strikes can rend and batter asunder the finest of armorsa testament to the harsher aspects of that tumultuous historical epoch. If you want to Engrave something like Name, Logo, Symbol, Image, Runes etc. We also accept Custom Personalized and Wholesale orders. WE DO ACCEPT CUSTOM PERSONALIZED & WHOLESALE ORDERS ![]()
![]() “Those spies would have been able to achieve what they were sent here for - to obtain technology and military secrets, to exert influence on American policy and policymakers. “The SVR’s goals are planned and scheduled,” Korczak said. ![]() This, he said, explains why the busted ring seems to have accomplished very little in the way of espionage. “My experience with the CIA was, ‘It has to be here, now, and make a big splash.’ The Russians will wait 15, 20 years - whatever.” ![]() “The Russians are very patient,” Korczak said. If they do, they are sent to the United States, almost always as sleepers. Once a spy seems suitably Americanized, they are sent to a way station - usually Finland or the Netherlands - where they attempt to pass as American. Russian spies-in-training in these towns, Korczak said, “buy groceries at 7-Elevens, eat hamburgers at McDonald’s, watch American TV and go to American movie theaters, get American newspapers delivered every morning and speak only English.” just outside Washington, where the bulk of KGB agents were deployed during World War II. They are exact replicas of American suburbs such as Chevy Chase, Md. SOME of these schools are located in small towns in the southern part of the country. “They’re called ‘worm-on-a-hook’ agents.” Korczak believes that Chapman (real name: Anya Kuschenko) was likely schooled by the SVR - Russia’s post-KGB intelligence agency - in the art of seduction.ĭuring the Cold War, “the Soviet Union had a number of schools that trained beautiful women how to lure and satisfy powerful, rich, American men, sexually and intellectually,” he said. He refused to comment.Ĭhapman, who dressed in designer clothes, drank in swank downtown spots such as the Thompson Hotel, had her hair dyed red every week (she’s a natural brunette) and got frequent mani-pedis, was suspected by acquaintances of working as a hooker to fund her expensive tastes. Once in New York, she allegedly began dating a politically connected businessman from New Jersey named Michael Bittan. ![]() They divorced in 2006, after she began spending a lot of time without him and with her “Russian friends” instead. In 2002, she married a British student named Alex Chapman, now 30. She is currently being held in solitary confinement in a federal prison in Brooklyn and her lawyer, Robert Baum, told The Post that she is “very unaware” of the media frenzy she’s sparked.Ĭhapman, a Russian native who once said her father was a high-ranking member of the KGB, spent her time in New York City circulating among rich and powerful men. “But there are more Russian spies here now than during the Cold War.”Īmong the 10 accused Russian spies taken into custody last week is Anna Chapman, 28, a flame-haired bombshell who, unsurprisingly, has attracted the most attention. “The current atmosphere in the US is that we’re having a love affair with Russia, that the Cold War is over,” agreed Eugene Poteat, a retired senior CIA operative who served from 1960-1990. “Out of 1,000 spies, one or two will perform, will get access to our nuclear secrets,” Korczak said. That’s because each mole is a long shot, and the Russians want to maximize their odds. “I would say there are a few thousand here,” said Boris Korczak, a former double agent who worked for the CIA, spying on the KGB from 1973-1980. The Russians are coming? The Russians are here.Īmerica is infested with more Russian spies than at any point in history, say former intelligence agents who spoke with The Post. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |